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61.
We examined the impact of Hydropsychidae caddisfly larvae on the incipient motion of two sizes of narrowly graded fine‐gravel (4–6 and 6–8 mm). This impact was assessed relative to the collective impact of other abiotic and biotic processes that are potentially important conditioning agents of fine‐gravels. Trays of gravel were placed in the River Soar, Leicestershire, UK, where they were colonized to natural densities by caddisfly larvae. Identical trays that were surrounded by a 250 µm mesh were also deployed, preventing colonization but allowing field conditioning of sediments, including minor reworking of grains and biofilm development. After 21 days in the river, trays were removed to a laboratory flume where grain entrainment stresses were established. In addition to the colonized and conditioned treatments, critical shear stresses were measured for identical sediments that were not placed in the river (laboratory gravels). Gravels that were colonized by Hydropsychidae required significantly greater shear stresses for entrainment than conditioned trays (≤ 0·002), however, there was no significant difference between conditioned and laboratory gravels. This implies that the presence of caddisfly can be a more important influence on fine‐gravel stability than some conditioning processes. Shields parameter was compared across treatments and across the two gravel size‐fractions using two‐way ANOVA. No significant differences or interactions were observed, indicating that 4–6 mm gravel was stabilized to a similar degree as 6–8 mm gravel by conditioning and colonization processes. Our results extend earlier studies in two important ways: (1) entrainment stresses were established for fine gravels that were colonized at natural densities, under natural stream conditions; and (2) the caddisfly effect was measured relative to both field‐conditioned and unconditioned laboratory controls. The temporal and spatial distribution of silk‐spinning caddisfly larvae suggests that they have the potential to influence fine‐sediment mobility in many rivers, worldwide. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   
62.
Here, we have presented a field device for sampling periphyton in lentic systems, with a tropical lowland wetland in Colombia as the model system. The periphyton sampler (PS) is composed of a central soft iron pole to which rectangular woody boxes and glass slides are attached to lateral iron axes, which can be distributed at different depths. Our PS can be used to evaluate changes in the richness and density of algal communities at different scales as well as in water quality studies.  相似文献   
63.
64.
Biofilm mediated intertidal sediments exhibit more complex erosional behaviour than abiotic systems. A major feature of intertidal systems is the exposure to repeated cycles of high and low shear created by tidal conditions and also less predictable episodic events, such as storms. There is very little information on how biofilm-forming communities respond to these conditions. In this study, the effects of both single and repeated-cycles of shear on the stability of newly developed bio-sedimentary beds was examined. Cleaned sand, without any potential biostabilization, was used as the control. For the single-cycle scenario, biofilms were incubated on a non-cohesive sandy bed under prolonged low shear periods varying between 5 and 22 days, after which erosional stress was applied. No significant biostabilization was observed for the youngest bio-sedimentary bed (after five days of low shear incubation). After 22 days, microbial communities were characterized by a firmly attached surface biofilm. To cause erosion, greater hydrodynamic stress (0.28 Pa) was required. The erosional behaviour of the underlying sand was also affected in that bedform ripples noted in the control system were no longer observed. Instead, a sudden ‘mass erosion’ took place (0.33 Pa). The one-cycle scenario indicated that significant biostabilization of sand only occurred after a relative long calm period. Under repeated cycles of stress (five days of low stress followed by high stress event and re-incubation, repeated for four cycles = 20 days), frequent cyclic disturbance did not degrade the system stability but enhanced biostabilization. The properties of the sub-surface sediments were also affected where erosion rates were further inhibited. We hypothesize that organic material eroded from the bed acted as a ‘biofilm precursor’ supporting the development of new biofilm growth. A conceptual framework is presented to highlight the dynamics of bio-sedimentary beds and the effects of growth history under repeated-cycles. © 2019 John Wiley & Sons, Ltd.  相似文献   
65.
为了研究全浸15天不锈钢表面生物膜的形成情况,首先采用SEM及荧光显微镜进行观察,显示全浸15天不锈钢表面已有大量微生物及其腐蚀产物附着,表明其表面生物膜已经形成;进一步通过EDS对不锈钢表面进行元素分析,发现实海全浸15天的316L不锈钢表面出现了大量组成生物体的C、P等基本元素,并且还出现了不锈钢基体中所不存在而存在于生物膜及其代谢产物中O等元素,进一步证明了不锈钢表面生物膜的形成。采用16S rRNA基因结合PCR-RFLP技术分析手段对实海全浸15天的316L不锈钢表面生物膜细菌群落的多样性进行了研究,发现316L不锈钢表面附着细菌的OTU数为24,分别属于变形菌门和拟杆菌门,其中α-变形菌的OTU数是18,占总克隆数的72.3%,为绝对优势种群。  相似文献   
66.
The vertical distribution of microphytobenthos (MPB) was investigated in intertidal flats of the Nakdong River estuary over different temporal periods. The MPB biomass were measured as chlorophyll a concentration in the sediment, and monitored at two different depth scales. From August 2006 to August 2007, monthly sampling was carried out at four sites to a depth of 10 cm. The vertical distribution exhibited an exponential decline with depth and the slope was closely related to sediment composition and MPB biomass in the surface sediment. The two slightly muddy sand (5–25% mud, <63 μm) sites presented similar trends of seasonal variation to a sediment depth of 10 cm, and no statistical difference was observed in their initial values and the slope of the exponential curves applied to each data set. The site with higher mud content also had higher surface biomass, and the fitted exponential curve had a larger slope than sites with more sand content. The seasonal variations of phaeo-pigments compared to chlorophyll a revealed higher grazing stress on the MPB in summer at the three slightly muddy sand sites, and low grazing stress all year around at the sand (<5% mud) site. In January, April and October 2007, and January 2008, diurnal sampling was conducted at hourly intervals at one slightly muddy sand site. The investigation in January 2007 which was conducted at 1 cm intervals to a depth of 10 cm sediment showed the migration mainly existed in the upper 1 cm. The other diurnal sampling was undertaken to a 1 cm depth at 0.5 mm intervals. An evident migratory rhythm was present, with migration up to the surface during daytime emersion and down into deeper sediment during night and high tide submersion. Migrating cells could reach sediments as deep as 6 or even 10 mm. When the visible thick biofilms formed, the migratory rhythm changed by keeping higher biomass at the surface even at night and during high tide submersion. Generally, the MPB surface biomass was an important factor in structuring the MPB vertical distribution. The vertical distribution to depths of 10 cm was closely related to the sediment composition over the long term, while the MPB migration influenced the vertical distribution over the short term in the top 1 cm of the sediment.  相似文献   
67.
The settlement substrates of nona-porous abalones (Haliotis diversicolor supertexta) are covered with biofilms in which several types of microorganisms coexist and interact. These microorganisms are usually important causes of juvenile abalone disease as well as organisms useful in promoting abalones’ adhesion. The bacterial community structure of the biofilms remains unclear. The aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria. Total DNA of b...  相似文献   
68.
THE APPLICATION AND RESEARCH PROGRESS OF BIOREMEDIATION IN POND AQUACULTURE   总被引:1,自引:0,他引:1  
邓来富  江兴龙 《海洋与湖沼》2013,44(5):1270-1275
当前我国池塘养殖多数仍沿用传统的池塘养殖模式, 普遍存在以下问题: 高密度养殖、饵料大量投喂、池塘水质恶化、病害频发等。然而传统的应对方法主要是大量换水, 由此产生的养殖废水直接排放到临近水域, 已导致污染扩散、水环境恶化、水域富营养化等系列问题。为降低池塘养殖面源污染, 确保池塘养殖业健康可持续发展, 本文介绍了目前运用于修复池塘养殖水环境的生物修复主要方法, 包括: 微生物修复法——微生态制剂和固定化微生物、植物修复、动物修复法、生物膜法、几种生物修复技术的综合运用等方法, 展望了生物修复方法在池塘养殖水环境修复方面的应用前景, 认为池塘养殖生物膜修复法因具有良好且稳定的水质改良、节能减排及增产增效等效果而具有广阔的应用推广前景。  相似文献   
69.
The effect of the overlying water velocity on ammonium (NH4+) uptake by benthic biofilms was studied in a recirculating laboratory flume (260 cm long, 29 cm wide), packed with 5 cm of silica sand arranged into bedforms. NH4+ uptake was determined as the reduction in NH4+ concentration in the water at average overlying water velocities of 0.8, 2, 4 and 8 cm s?1. NH4+ uptake was relatively constant under laminar flow conditions but increased when the flow regime became turbulent (>4 cm s?1). This pattern was observed for two biofilms differing in their total biomass and in the abundance of the ammonia‐oxidizing bacteria, thus indicating that NH4+ uptake was strongly controlled by mass‐transfer processes. The near stoichiometric relationship between the rates of NH4+ uptake and nitrate (NO3?) accumulation suggests that aerobic nitrification was the main route for NH4+ uptake. Microelectrode measurements showed a sharp decline of oxygen concentrations and pH values within the biofilms, thus supporting strong nitrification activity within the surficial section of the benthic biofilms. The results of this study highlight the key role of hydrodynamic conditions in regulating NH4+ uptake in the transition from laminar to turbulent flow conditions. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   
70.
海洋生物膜的形成及其对金属腐蚀的影响   总被引:3,自引:1,他引:2  
许昆明 《海洋科学》2008,32(9):71-75
为了揭示海洋生物膜形成对金属腐蚀的影响,描述了在不锈钢上形成的海水生物膜,制备了伏安微电极,并测量生物膜中氧化还原化学成分如溶解氧、过氧化氢、锰和硫在不同位置的垂直分布。结果表明,生物膜中存在溶解氧的垂直浓度梯度。在溶解氧浓度小于10μmol/L条件下,生物膜中的某些位置出现过氧化氢和锰离子,在无氧条件下,生物膜中有硫化物出现,这些化学成分表明生物膜中确实有锰氧化还原菌或者硫酸盐还原菌的活动迹象。生物膜在固体表面的分布是不均匀的,浓差电池或者生物极化作用的存在意味着生物膜的形成可能加速金属基体表面的局部腐蚀过程。  相似文献   
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